lambda fixii 129svj mouse genomic library Search Results


pgl3-basic vector  (Promega)
90
Promega pgl3-basic vector
(A) Schematic diagram of the promoter-luciferase fusion plasmids is shown on the left, where the 5′/3′ ends of the construct relative to the transcription initiation site are indicated. Each construct was transiently transfected into P53LMACO1 (closed bar) or MCE301 cells (opened bar). The β-galactosidase-expression vector was co-transfected as an internal control. The relative luciferase activity was denoted as the ratio to the activity of the <t>pGL3-basic</t> vector. Bars represent means ±SE of at least three experiments.
Pgl3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+fixii+129svj+mouse+genomic+library/pmc01550311-142-31-33?v=Promega
Average 90 stars, based on 1 article reviews
pgl3-basic vector - by Bioz Stars, 2026-07
90/100 stars
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(A) Schematic diagram of the promoter-luciferase fusion plasmids is shown on the left, where the 5′/3′ ends of the construct relative to the transcription initiation site are indicated. Each construct was transiently transfected into P53LMACO1 (closed bar) or MCE301 cells (opened bar). The β-galactosidase-expression vector was co-transfected as an internal control. The relative luciferase activity was denoted as the ratio to the activity of the pGL3-basic vector. Bars represent means ±SE of at least three experiments.

Journal:

Article Title: Novel transcripts of Nox1 are regulated by alternative promoters and expressed under phenotypic modulation of vascular smooth muscle cells

doi: 10.1042/BJ20060300

Figure Lengend Snippet: (A) Schematic diagram of the promoter-luciferase fusion plasmids is shown on the left, where the 5′/3′ ends of the construct relative to the transcription initiation site are indicated. Each construct was transiently transfected into P53LMACO1 (closed bar) or MCE301 cells (opened bar). The β-galactosidase-expression vector was co-transfected as an internal control. The relative luciferase activity was denoted as the ratio to the activity of the pGL3-basic vector. Bars represent means ±SE of at least three experiments.

Article Snippet: The 5′-flanking regions of the corresponding exons for the identified transcripts were isolated from a mouse genomic library (Lambda FIXII Mouse 129/SVJ, Stratagene, La Jolla, CA, U.S.A.), and cloned into a pGL3-basic vector (Promega).

Techniques: Luciferase, Construct, Transfection, Expressing, Plasmid Preparation, Activity Assay